The (Epi)Transcriptomic core unit in IPEK houses a complete next-generation sequencing (NGS) workflow and bioinformatic expertise supporting a wide range of methods such as transcriptome sequencing, single-cell profiling, and epigenome applications. This unit operates an Illumina NextSeq sequencing system, the 10X genomics Chromium X controller for single-cell resolution, as well as equipment for chromatin/RNA shearing (Bioruptor Pico) and quality-check of input samples and DNA libraries.

The main applications include:

•  Stranded mRNA sequencing
• -Stranded total RNA sequencing
• -Small RNA sequencing
• -Chromatin Immunoprecipitation and sequencing (ChIP-seq)
• -Single-cell genomic applications (e.g., scRNAseq)