Flow cytometric analysis of cellular phenotypes in complex cell mixtures has become a gold-standard method for addressing diverse research questions in cellular and molecular biology. Beyond its traditional role in assessing surface molecules, modern flow cytometry enables measurement of enzymatic activities, analysis of cell cycle dynamics, evaluation of signaling cascade activation, assessment of organelle function and production of secreted molecules.
Our Shared Resource Lab offers advanced experimental expertise and state-of-the-art technology for analytical multicolor flow cytometry and cell sorting. These services are available to all research groups within the Institute, supporting cutting-edge analysis and sorting of a diverse range of cell types.
INSTRUMENTATION
The Institute hosts three cutting-edge flow cytometers and one advanced cell sorter, enabling precise detection and analysis of cells in tissues.
- BD FACSCanto II
Features: Detection of up to 10 parameters simultaneously
- BD LSRFortessa X-20
Features: Detection of up to 20 parameters simultaneously
- BD FACSymphony A5
Features: Detection of up to 30 parameters simultaneously
- BD FACSAria III
Features: Detection of up to 12 parameters
Aseptic bulk or single-cell sorting with high purity and viability
Capable of sorting cells with multiple markers at varying expression levels
Expert operator maintenance ensures optimal performance for downstream applications
APPLICATIONS
The Shared Resource Lab specializes in the analysis and sorting of various cell types, including:
- Immune cell subsets (neutrophils, monocytes, macrophages, dendritic cells, NK cells, T cells, and B cells)
- Hematopoietic progenitor cells and platelets
- Cells derived from tissue/organ homogenates
- Transduced cell lines and apoptotic microvesicles
CONTACT INFORMATION
- For BD LSRFortessa X-20 and BD FACSymphony A5, please contact Johan Duchêne
- For BD FACSAria III and BD FACSCanto II, please contact Michael Hristov